Detection by Culture and by Multiplex Real-Time PCR of Staphylococcus aureus and Staphylococcus spp. in Vaginal Secretions and Urines in Patients Received at Saint Camille Hospital of Ouagadougou, Burkina Faso
Rabiétou Nikiema *
Faculty of Sciences and Technologies, Saint Thomas Aquinas University, 03 BP 702 1 Ouagadougou O3, Burkina Faso.
Théodora M. Zohoncon
Faculty of Sciences and Technologies, Saint Thomas Aquinas University, 03 BP 702 1 Ouagadougou O3, Saint Camille Hospital of Ouagadougou, Burkina Faso.
Prosper Bado
Pietro Annigoni Biomolecular Research Center (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.
Geraud Joel Guigma
Pietro Annigoni Biomolecular Research Center (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso.
Amana Metuor Dabiré
University of Dédougou, BP 176 Dédougou, Laboratory of Molecular Biology and Genetics (LABIOGENE) UFR-SVT, Joseph KI-ZERBO University, 03 BP 702 1 Ouagadougou 03, Burkina Faso.
Jacques Simpore
Faculty of Sciences and Technologies, Université Saint Thomas d’Aquin, Pietro Annigoni Biomolecular Research Center (CERBA), Saint Camille Hospital of Ouagadougou, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Staphylococci are bacteria involved in various pathologies and varying degrees of severity. They are one of the first causative agents of nosocomial and community infections with humans and animals as natural habitats. The genus Staphylococcus is subdivided into two groups, coagulase-negative staphylococci, the most isolated of which is Staphylococcus aureus and coagulase-positive staphylococci and constitutes about fifty species and subspecies. Some of these species are implicated in urinary tract and vaginal tract infections and are a real health problem especially for women. The aim of the study was to detect S. aureus and Staphylococcus spp. in vaginal secretions and urine using culture and real-time PCR. The microbiological analysis was done using the conventional methods adopted at Saint Camille's hospital laboratory. Molecular analysis was done using the Sacace multiplex PCR kit.
We examined a total of 97 samples including 77 urine samples and 20 vaginal secretion samples. At culture, no Staphylococcus was isolated. On the other hand, with the same culture-negative samples, we were able to identify two S. aureus and two Staphylococcus spp. in the urine and three Staphylococcus spp in vaginal secretions. The study then demonstrates the difference in sensitivity between the two identification techniques and confirms that there is a possibility of infection in case of negative culture. It is then necessary to involve rt-PCR in routine analyses.
Keywords: Real-time multiplex PCR, culture, S. aureus, Staphylococcus spp, Burkina Faso