In vitro Sterilisation of Different Explants of Chrysanthemum (Dendranthemum morifolium L.) cvs. “Candid” and “Flirt”
Ambreena Din *
Division of Floriculture and Landscape Architecture, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir, India.
Z. A. Qadri
Division of Floriculture and Landscape Architecture, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir, India.
Z. A. Rather
Division of Floriculture and Landscape Architecture, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir, India.Q
M. Saleem. Mir
Associate Directorate of Research, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir, India.
Imtiyaz Murtaza
Divison of Biochemistry, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir, India.
F. A. Khan
Division of Basic Science and Humanities, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir, India.
Neelofar .
Division of Floriculture and Landscape Architecture, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir, India.
Muneeb Ahmad Wani
Division of Floriculture and Landscape Architecture, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Jammu and Kashmir, India.
*Author to whom correspondence should be addressed.
Abstract
The study was conducted to standardise protocol for sterilisation of different explants of chrysanthemum cvs. “Candid and “Flirt”. Different explants tried were shoot tips, petals, nodal segments and leaf. Carbendazim 200 ppm for 30 minutes + mercuric chloride (0.1%) dip for 2 minutes and final treatment with ethyl alcohol (70%) for 10 seconds proved best for culture asepsis of shoot tips followed by petals, nodal segments and leaf segments in both the cultivar. Carbendazim 200 ppm for 30 minutes + mercuric chloride (0.1%) dip for 2 minutes and final treatment with ethyl alcohol (70%) for 10 seconds proved better sterilisation for culture survival of shoot tips followed by petals, nodal segments and leaf segments in cv. “Candid” whereas, petals followed by leaf segments in case of cv. “Flirt”. This study leads to the standardisation of the shoot tip and petal explants for culture asepsis and survival in chrysanthemum cvs. “Candid” and “Flirt” and among two cultivars "candid" proved better in sterilisation than "Flirt".
Keywords: Tissue culture, in vitro sterilisation, asepsis, survival, chrysanthemum, Candid and Flirt.