Shelf Life Extension of Banana (Musa spp.) using Hexanal Formulation as a Post-harvest Dip

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I. Muthuvel
S. Srivignesh
P. Mutharasu
M. Kavino
K. S. Subramanian


Postharvest dipping of fruits in hexanal formulation extends the shelf life by inhibiting enzyme phospholipase D (PLD) activity. To enhance the postharvest shelf-life and quality of different banana cultivars viz., Grand Naine (AAA), Ney Poovan (AB), Poovan (AAB) and Rasthali (AAB) were treated with different concentration of hexanal formulations (1% and 2% Enhanced fresh formulation (EFF)) and stored at atmospheric storage condition. The results indicate that the quality characteristics of 2% EFF treated fruits of Grand Naine (AAA), Poovan (AAB) and Rasthali (AAB) were low TSS, total sugars, reducing sugars and acidity indicating the perpetuation in quality of fruits during storage life besides extended shelf life of fruits. Banana fruits treated with hexanal, irrespective of cultivars experienced a significant delay in weight loss and higher firmness as compared to respective control.  The high-resolution imaging had clearly shown that the application of hexanal delayed the ripening process by the structural integrity of cells on skin and fruit pulp. The PLD enzyme activity, respiration and ethylene evolution rates (more than 10%) were markedly reduced over control due to hexanal treatment. Our results revealed that the application of hexanal formulation as postharvest dip treatment in different banana cultivars significantly enhanced shelf life over control besides continuance of quality during the storage period.

Enhanced freshness formulation, structural changes, Phospholipase D enzyme (PLD), storage.

Article Details

How to Cite
Muthuvel, I., Srivignesh, S., Mutharasu, P., Kavino, M., & Subramanian, K. S. (2019). Shelf Life Extension of Banana (Musa spp.) using Hexanal Formulation as a Post-harvest Dip. Current Journal of Applied Science and Technology, 38(6), 1-12.
Original Research Article


Food and Agriculture Organization of the United Nations. FAOSTAT Statistical Database. [Rome] :FAO; 2017.

Ministry of Agriculture and Farmers Welfare. Horticultural Statistics at a Glance 2017. Horticulture Statistics Division, Department of Agriculture, Cooperation & Farmers Welfare, Ministry of Agriculture & Farmers Welfare, Government of India. 2017;1-481.

Liu X, Shiomi S, Nakatsuka A, Kubo Y, Nakamura R, Inaba A. Characterization of ethylene biosynthesis associated with ripening in banana fruit. Plant physiology. 1999;121(4):1257-1265.

Sakyi‐Dawson E, Asamoah‐Bonti P, Amponsah Annor G. Biochemical changes in new plantain and cooking banana hybrids at various stages of ripening. Journal of the Science of Food and Agriculture. 2008;88:2724-2729.

Saúco VG, Robinson JC. Field establishment of in vitro-produced banana plants. Fruits 2010; 65:43-51.

AGMARKNET: NICNET Based Agricultural Marketing Information System Network - A Sponsored Project of the Directorate of Marketing and Inspection (Ministry of Agriculture); 2015. Available:

Dek MSP, Padmanabhan P, Subramanian J, Paliyath G. Inhibition of tomato fruit ripening by 1-MCP, wortmannin and hexanal is associated with a decrease in transcript levels of phospholipase D and other ripening related genes. Postharvest Biology and Technology. 2018; 140:50-59.

Sharma M, Jacob JK, Subramanian J, Paliyath G. Hexanal and 1-MCP treatments for enhancing the shelf life and quality of sweet cherry (Prunus avium L.). Scientia Horticulturae 2010;125:239-247.

Cheema A, Padmanabhan P, Subramanian J, Blom T, Paliyath G. Improving quality of greenhouse tomato (Solanum lycopersicum L.) by pre-and postharvest applications of hexanal-containing formulations. Postharvest Biology and Technology. 2014;95:13-19.

Anusuya P, Nagaraj R, Janavi GJ, Subramanian KS, Paliyath G, Subramanian J. Pre-harvest sprays of hexanal formulation for extending retention and shelf-life of mango (Mangifera indica L.) fruits. Scientia Horticulturae. 2016;211:231-240.

Gill K, Dhaliwal H, Mahajan B, Paliyath G, Boora R. Enhancing postharvest shelf life and quality of guava (Psidium guajava L.) cv. Allahabad Safeda by pre-harvest application of hexanal containing aqueous formulation. Postharvest Biology and Technology. 2016;112:224-232.

Kumar SK, El Kayal W, Sullivan JA, Paliyath G, Jayasankar S. Pre-harvest application of hexanal formulation enhances shelf life and quality of ‘Fantasia’nectarines by regulating membrane and cell wall catabolism-associated genes. Scientia Horticulturae. 2018;229:117-124.

Paliyath G, Subramanian J. Phospholipase D inhibition technology for enhancing shelf life and quality. In: Paliyath G, Murr DP, Handa AK, Lurie S. (Eds.), Postharvest Biology and Technology of Fruits, Vegetable, and Flowers., 1st Ed. Wiley-Blackwell, USA. 2008;195–239.

Jincy M, Djanaguiraman M, Jeyakumar P, Subramanian K, Jayasankar S, Paliyath G. Inhibition of phospholipase D enzyme activity through hexanal leads to delayed mango (Mangifera indica L.) fruit ripening through changes in oxidants and antioxidant enzymes activity. Scientia Horticulturae. 2017;218:316-325.

Archbold Hamilton-Kemp T, Barth M, Langlois B. Identifying natural volatile compounds that control gray mold (Botrytis cinerea) during postharvest storage of strawberry, blackberry, and grape. Journal of Agricultural and Food Chemistry. 1997;45:4032-4037.

Lanciotti R, Corbo MR, Gardini F, Sinigaglia M, Guerzoni ME. Effect of hexanal on the shelf life of fresh apple slices. Journal of Agricultural and Food Chemistry. 1999;47:4769-4776.

Archbold, Hamilton-Kemp TR, Clements AM, Collins RW. Fumigating Crimson Seedless Table Grapes with (E)-2-Hexenal Reduces Mold during Long-term Postharvest Storage. Hort Science 1999;34:705-707.

El Kayal W, Paliyath G, Sullivan JA, Subramanian J. Phospholipase D inhibition by hexanal is associated with calcium signal transduction events in raspberry. Horticulture research 2017; 4:17042.

Jha SN, Jaiswal P, Narsaiah K, Kaur PP, Singh AK, Kumar. Textural properties of mango cultivars during ripening. Journal of Food Science and Technology. 2013;50:1047-1057.

Ranganna S. Handbook of analysis and quality control for fruit and vegetable products. Tata McGraw-Hill Education; 1986.

Association of Office Analytical Chemists. Official Methods of Analysis, 15th Ed.George Banta, Washington DC; 1996.

Hedge JE, Hofreiter BT. Methods in Carbohydrate Chemistry Volume 17. In: Whistler RL, BeMiller JN. (Eds.). Academic Press, New York. 1962;420.

Nelson N. A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 1944;153:375-380.

Kitsaki C, Vemmos S, Tzoutzoukou C. Changes of respiration rate, ethylene evolution, and abscisic acid content in developing inflorescence and young fruit of olive (Olea europaea L. cv.konservolia). Journal of Plant Growth Regulation. 1999;18:1-7.

Thompson AK. Controlled atmosphere storage of fruits and vegetables. CABI; 2010.

Siriboon N, Banlusilp P. A study on the ripening process of ‘Namwa’ banana. AU Journal of Technology. 2004;7:159-164.

Dharmasena D, Kumari A. Suitability of charcoal-cement passive evaporative cooler for banana ripening. The Journal of Agricultural Sciences. 2005;1:19-31.

Ashwini T, Ganapathy S, Subramanian K, Rani CI, Meenakshi GG. Effect of Hexanal Vapour on Postharvest Quality and Shelf Life of Banana var. Grand Naine. Int. J. Curr. Microbiol. App. Sci 2018;7:2441-2450.

Shiga TM, Fabi JP. do Nascimento JORO, Petkowicz CLCDO, Vriesmann LCC, Lajolo FM, et al. Changes in cell wall composition associated to the softening of ripening papaya: Evidence of extensive solubilization of large molecular mass galactouronides. Journal of agricultural and food chemistry. 2009;57:7064-7071.

Thumdee S, Manenoi A, Chen NJ, Paull RE. Papaya fruit softening: role of hydrolases. Tropical Plant Biology. 2010;3:98-109.

Ali A, Muhammad MTM, Sijam K, Siddiqui Y. Effect of chitosan coatings on the physicochemical characteristics of Eksotika II papaya (Carica papaya L.) fruit during cold storage. Food Chemistry. 2011;124:620-626.

Cheema A, Padmanabhan P, Amer A, Parry MJ, Lim LT, Subramanian J, et al. Postharvest hexanal vapor treatment delays ripening and enhances shelf life of greenhouse grown sweet bell pepper (Capsicum annuum L.). Postharvest Biology and Technology. 2018;136:80-89.

Nandhini P. Effect of hexanal treatment to extend the shelf-life of guava (Psidium guajava L.), Department of Fruit Crops, TNAU, HC&RI, Periyakulam; 2017.

Venkatachalam K, Muthuvel I, Sundaresan S, Subramanian KS, Janaki JG, Sullivan JA, et al. Post-harvest dip of enhanced freshness formulation to extend the shelf life of banana (Musa acuminata cv. Grand Naine) in India. The Journal of the Faculty of Food and Agriculture. 2018; 95(1):1-13

Jeffery D, Smith C, Goodenough P, Prosser I, Grierson D. Ethylene-independent and ethylene-dependent biochemical changes in ripening tomatoes. Plant physiology. 1984;74:32-38.

Jayaraman J. Laboratory manual in biochemistry. Wiley Eastern; 1981.

Pinhero RG, Almquist KC, Novotna Z, Paliyath G. Developmental regulation of phospholipase D in tomato fruits. Plant Physiology and Biochemistry. 2003;41:223-240.

Bai XH, Teng LH, Lu DQ, Qi HY. Co-treatment of EFF and 1-MCP for enhancing the shelf-life and aroma volatile compounds of oriental sweet melons (Cucumis melo var. makino). Journal of Integrative Agriculture. 2014;13:217-227.

Morrelli K, Kader A. Recommendations for maintaining postharvest quality. Department of pomology, University of California, Davis California;2002.

Lima LCDO, Chitarra AB, Chitarra MIF. Changes in amylase activity starch and sugars contents in mango fruits pulp cv. Tommy Atkins with spongy tissue. Brazilian Archives of Biology and Technology. 2001;44:59-62.

Dadzie BK, Orchard JE. Routine post-harvest screening of banana/plantain hybrids: criteria and methods. International Plant Genetic Resources Institute (IPGRI); 1997.

Lelièvre JM, Latchè A, Jones B, Bouzayen M, Pech JC. Ethylene and fruit ripening. Physiologia Plantarum. 1997;101:727-739.

Rothan C, Nicolas J. High CO2 levels reduce ethylene production in kiwifruit. Physiologia Plantarum. 1994;92:1-8.

Mathooko FM. Regulation of respiratory metabolism in fruits and vegetables by carbon dioxide. Postharvest Biology and Technology. 1996;9:247-264.

Paliyath G, Yada R, Muir DP, Pinhero RG. Inhibition of phospholipase D. US patent no 6,514,914; 2003.